Siti Aminah Ahmed

About Me

Expertise

•    Basic molecular techniques (PCR, cloning & sequencing).
•    Plant tissue culture and transformation.
•    Animal tissue culture (including A549, BHK, Vero, HT29, 3T3 and PS Clone D cell)
•    Basic cell morphology studies (fluorescence microscopy)
•    SDS-PAGE, Western blot, ELISA
•    Expression system including bacterial, baculovirus, plant and vaccinia
•    Cytotoxicity assay on herbal products.
•    Next Generation Sequencing data analysis using open software

Publication

SELECTED PUBLICATIONS 

Kanniappan P, Siti Aminah Ahmed, Rajasekaram G et al. RNomic identification and evaluation of npcTB_6715, a non-protein coding RNA gene as a potential biomarker for the detection of M. tuberculosis. J Cell Mol Med. (In-press)

Siti Aminah Ahmed, Lee Li Pin, Carsten A. Raabe et al. A combined rapid DNA extraction and multiplex PCR for the detection of porcine DNA in raw and processed food. In Contemporary Issues and Development in the Global Halal Industry: Selected papers from the International Halal Conference 2014. (Eds: Siti Khadijah Ab. Manan, Fadilah Abd Rahman, Mardhiyyah Sahri). Springer 2016. ISBN 9811014523.

Siti Aminah Ahmed, Hong Leong Cheah, Nithya Ravichantar et al. Evaluation of Palm PCR™G1-12 System: a portable battery-operated PCR thermal cycler. Songklanakarin J. Sci. Technol. 38(4), 343-348.

Ravichantar Nithya, Siti Aminah Ahmed, Chee-Hock Hoe et al. Non-protein coding RNA genes as the novel diagnostic markers for the discrimination of Salmonella species using PCR. PLoS ONE 10(3): e0118668. doi:10.1371/journal.pone.0118668

Thean-Hock Tang, Siti Aminah Ahmed, Mustaffa Musa & Zainul Fadziruddin Zainuddin. Rapid detection of Mycobacterium tuberculosis in clinical samples by multiplex polymerase chain reaction (mPCR). World J Microbiol Biotechnol. doi: 10.1007/s11274-013-1407-0.

Siti Aminah Ahmed, Doblin Anak Sandai, Suzana Musa et al. Rapid Diagnosis of Leptospirosis by Multiplex PCR. Malays J Med Sci 19(3):9-16.

Research Grant

RESEARCH GRANT (ACTIVE):

2016-2019; Transdisciplinary Research Grant Scheme, MOHE; Investigation of Bio-characterization, cytotoxicity and antimicrobial activity of nanocatalyst/polypropylene (PP) nanocomposite for bio-medical applications; (Co-researcher) 

2015-2018; ScienceFund, MOSTI; Tracing porcine DNA in processed food: A strategy for halal food quality control; (Principle investigator)

2015-2018; Fundamental Research Grant Scheme, MOHE; The role of small RNA (sRNA) in pathogenesis and virulence of Leptospira: a transcriptomic approach; (Co-researcher)

2015-2017; Short Term Grant, USM; A combined rapid DNA extraction and multiplex PCR for the detection of porcine DNA in meat-based food products; (Principle investigator)

Academic Recognition

Journal Reviewer:

 BioMed Research International

 Jurnal Teknologi 2018 (Special Edition)

Professional bodies: 

 International Leptospirosis Society, ILS. (Life member)

International Society of Infectious Diseases, ISID. (Life member)

Awards: 

 International Penang Invention, Innovation and Research Design Platform (PIID 2017) – Gold Medal

 International Penang Invention, Innovation and Research Design Platform (PIID 2017) – Silver Medal

 International Conference and Exposition on Inventions by Institutions of Higher Learning (PECIPTA '15) – Bronze Medal 

 Most Active Research Officer, AMDI, USM (2014)

 24th International Invention, Innovation and Technology Exhibition (ITEX '13) – Gold Medal 

 International Invention, Innovation and Research Design Platform (IP 2013) – Gold Medal

 International Invention, Innovation and Research Design Platform (IP 2013) – Silver Medal

Patents:

 National Patent No. PCT/MY2015/050016; A method of detecting porcine markers for identifying pork  content in a sample.