Associate Professor Dr. Tan Mei Lan

About Me

Biostatment: Mei Lan Tan is Associate Professor at the Advanced Medical and Dental Institute, Universiti Sains Malaysia and a Senior Research Fellow of the Malaysian Institute of Pharmaceuticals and Nutraceuticals (IPharm), NIBM. Her main interest is drug-herb and drug-food interaction studies in various aspects such as the P450 cytochromes, membrane transporters (P-glycoprotein and organic transporters) and hERG potassium channel blockade. She hopes to provide extensive safety information and drug-herb-food interaction risks data on local food and herbal components to promote well-being and appropriate use of supplements and herbal medicine. Her other interests include studying the fundamental relationships between different types of cell death elicited by natural compounds, with much interest in Autophagy and ER-stress.

Education

Expertise

Molecular Pharmacology (Cell Death and Autophagy) 

Mechanistic Toxicology (Drug-Drug and Drug-Herb Interactions)

Publications

Proceedings and Abstracts

1. Choy KC, Baharuddin A, Lim CH, Tan ML, Najimudin N, Samian MR, Tengku Muhammad TS. Amplification of the regulatory region of peroxisome prooliferator activated receptor gamma 2 from bovine. 12th National Biotechnology Seminar, 12-15th November 2000, Lumut, Perak.

2. Tan ML, Najimudin N, Sulaiman SF, Tengku Muhammad TS. Cytotoxic effects of Pereskia corrugata extracts against different classes of cell lines. Second Asia Pacific Conference on Chemical Ecology, “Current scenario and trends in chemical ecology”. August 7-11, 2001, Penang.

3. Tan ML, Najimudin N, Sulaiman SF, Tengku Muhammad TS. Growth inhibition and apoptosis of breast carcinoma cell line by Pereskia corrugata extracts. The 13^th National Biotechnology Seminar, “Towards commercialization of Malaysian Biotechnology. 10-13^th November, 2001, Penang, Malaysia.

4. Tan ML, Najimudin N, Sulaiman SF, Tengku Muhammad TS. Growth inhibition and apoptosis of breast carcinoma cell line by Pereskia corrugata extract. The Annual Workshop National Science Fellowship (NSF). 14-15 January, 2002, Malaysia (oral presentation)

5. Tan ML, Najimudin N, Sulaiman SF, Tengku Muhammad TS. Cytotoxic effects of Murdannia lineolatum extracts against different classes of cell lines. The Fourth Regional IMT-GT UNINET Conference, “Future scenario in biological research insights and co-operation. 15-17^th October, 2002, Malaysia.

6. Tan ML, Najimudin N, Sulaiman SF, Tengku Muhammad TS. Growth arrest and non-apoptotic cell death associated with the up-regulation of c-myc expression in T-47D breast carcinoma cells following exposure to Epipremnum pinnatum hexane extract. International Seminar & Workshop on Natural Products. 13-16 October 2003, Kuala Lumpur, Malaysia.

7. Tan ML, Najimudin N, Sulaiman SF, Tengku Muhammad TS. Cytotoxic activities of seven bioactive compounds of Andrographis paniculata against a panel of five human tumor cell lines: 14-deoxy-11,12-didehydro-andrographolide induces a non-apoptotic programmed cell death in T-47D, a breast carcinoma cell line. 14^th National Biotechnology Seminar, “DNA: 50 Years and Beyond”. 11 – 13^th December, 2003, Penang, Malaysia. (Oral presentation, Best & Outstanding Poster Presentation Award).

8.Oon CE, Tengku Muhammad TS, Sulaiman SF, Tan ML. Differential gene expression patterns in T-47D mammary carcinoma cells undergoing Type II (autophagic) programmed cell death. 9th Malaysian Applied Biology Symposium. 30-31 May 2007, Penang.

9.Marpaung L, Tan ML, Tengku Muhammad TS, Lim BP, Wong KC. Cytotoxic activities of flavanone compounds from fruits of Macaranga gigantea M. A. 9th Malaysian Applied Biology Symposium. 30-31 May 2007, Penang.

10. Mutee AF, Kaur G, Khalid IA, Kumar G, Ashman, JE, Hassan MS, Tengku Muhammad TS, Tan ML.. Immunohistochemical expression of autophagy-related mTOR and Beclin-1 protein in breast cancer tissues, a preliminary study. 24th World Congress of Pathology and Laboratory Medicine, Malaysia. 20-24 August 2007.(Published in the Malaysian Journal of Pathology, Vol 29, Supp A, Aug 2007).

11. Tan ML, Oon CE, Mutee AF, Sulaiman SF, Tengku Muhammad TS.. Rapamycin-induced autophagy in T-47D mammary cells revealed novel gene expression patterns. Cell Death Meeting, 30 September 2007. Cold Spring Harbor Laboratory New York, USA.

12.Ooi JP, Kuroyanagi M, Tengku Muhammad TS, Sulaiman SF, Tan ML. The effects of diterpenes from Andrographis paniculata Nees on the cytochrome P450 gene expression patterns in HepG2 cells, elucidating potential drug-herb interactions, 4th Regional Conference on Molecular Medicine 2009, 2^nd May – 4^th May 2009 Kelantan, Malaysia.

13.Ismail NI, Yusoff NM, Tengku Muhammad TS, Tan ML. The effects of cytokines on the mTOR signaling pathway and autophagy related genes in U937 leukemia cell line. 4th Regional Conference on Molecular Medicine 2009, 2^nd -4^th May 2009, Kelantan, Malaysia.

14.Moad AI, Tengku Muhammad TS, Tan ML. Up-regulation of PHLDA1 gene expression in rapamycin-induced autophagy in T47D breast carcinoma cells. 4th Regional Conference on Molecular Medicine 2009, 2^nd -4^th May 2009, Kelantan, Malaysia.

15. Ismail NI, Yusoff NM, Tengku Muhammad TS, Tan ML. The effects of cytokines on the mTOR signaling pathway in Kasumi-1 leukemia cell line. BIT Life Sciences’ 2^nd World Cancer Congress 2009, Beijing, China. 22^nd -25^th June 2009.

16. Tan ML Immunohistochemical expression of MAP1LC3A and MAP1LC3B protein in breast carcinoma tissues. National Biotechnology Seminar, 24-26 Mei 2010, PWTC Kuala Lumpur (oral presentation).

17. Utar Z, Majid MIA, Adenan IM, Tan ML. The effects of mitragynine on the expression of Cox-1 and Cox-2 in LPS-stimulated RAW264.7 macrophage cells. The Consortium for Globalization of Chinese Medicine, 23^rd -25^th August , 2010, Hong Kong Convention and Exhibition Centre, Hong Kong.

18.Moad AI, Tengku Muhammad TS, Tan ML. CTSB is involved in the molecular mechanism of rapamycin-induced autophagy in T-47D breast carcinoma cells. 1^st AMDI International Biohealth Science Conference (IBSC) 2010. 29 Nov – 1^st Dec 2010, Penang, Malaysia.

19. Koe XF, Tengku Muhammad TS, Chong, SC, Adenan MI, Tan ML. Multiplex Real Time Reverse Transcription Polymerase Chain Reaction for the simultaneous detection of CYP1A2, CYP2D6 and CYP3A4 mRNA expression level. Asian Regional Conference on Systems Biology, 29^th Nov – 1^st Dec 2010, Kuala Lumpur.

20. Seah TC, Tengku Muhammad TS, Chong SC, Adenan MI, Tan ML. Construction of stable HepG2 cell expressing human CYP3A4 promoter tagged with green fluorescence protein (GFP). Asian Regional Conference on Systems Biology, 29^th Nov – 1^st Dec 2010, Kuala Lumpur.

21.Koe XF, Tengku Muhammad TS, Chong SC, Adenan MI, Tan ML. Detection of CYP1A2, CYP2D6, and CYP3A4 in a Multiplex Real-time Reverse Transcription Polymerase Chain Reaction (qRT - PCR) Assay. qPCR Europe, 8-9 September 2011, Munich, Germany.

22. Seah Tiong Chai, Tengku Sifzizul Tengku Muhammad, Alexander Chong Shu-Chien, Mohd Ilham Adenan, & Tan Mei Lan. Development of cell-based GFP assay to assess the induction and inhibition of CYP3A4. 4^th Regional Conference on Molecular Medicine, 9-11^th October 2011, Pulau Pinang (Best Overall Work/International Prize)

23. Tan HK, Kuroyanagi M, Tan ML 14-deoxy-11,12 didehydroandrographolide induces cell death in T-47D breast carcinoma cells and causes feedback activation of mTOR. 4^th Regional Conference on Molecular Medicine, 9-11^th October 2011, Pulau Pinang.

24.Wan Mohamad WF, Tengku Muhammad TS, Jamil MF, Tan ML. Deoxyelephantopin (ESD) inhibits the component of mammalian target of rapamycin (mTOR) signaling pathway and induces apoptosis in MCF-7 cell line. 4^th Regional Conference on Molecular Medicine, 9-11^th October 2011, Pulau Pinang.

25. Lim EL, Tengku Muhammad TS, Wahab HA, Jamil MF, Tan ML. The effect of mitragynine on the metabolism of testosterone on the human CYP3A4 enzyme activity. 4^th Regional Conference on Molecular Medicine, 9-11^th October 2011. Pulau Pinang.

26. Tan HK, Kuroyanagi M, Tan ML. 14-deoxy-11,12-didehydroandrographolide induces activation of mTOR through a PI3K-independent feedback loop in T-47D cells. International Conference on Natural Products 2011, 14-16th November 2011, Putrajaya, Selangor.

27. Wan Mohamad WF, Tengku Muhammad TS, Jamil MF, Tan ML. Deoxyelephantopin (ESD) inhibits the component of Mammalian Target of Rapamycin (mTOR) signalling pathway and induces apoptosis in MCF-7 cell line. International Conference on Natural Products, ICNP 2011, 14-16^th November 2011, Putrajaya, Selangor.

28.Lim EL, Wahab HA, Amanah A, Tengku Muhammad TS,Tan ML The effect of deoxyelephantopin (ESD) on the metabolism of testosterone by the human CYP3A4 enzyme. International Conference on Natural Products 2011, 14-16^th November 2011, Putrajya, Selangor.

29. Seah TC, Tengku Muhammad TS, Chong SC, Adenan MI, Tan ML. Development of cell-based green fluorescence protein reporter gene assay to assess the induction and inhibition of human CYP3A4 by natural compounds. International Conference On Natural Products, 14-16^th November 2011, Putrajaya, Selangor.

30. Lim EL, Koe XF, Seah TC, Jamil MF, Adenan MI, Tan ML The effects of mitragynine on the human cytochrome P450 mRNA, protein and enzymatic activities. The 4th International Conference on Natural Products for Health and Beauty, 28th to 30th November 2012, Chiang Mai, Thailand (oral presentation)

31. Tan HK, Kuroyanagi M and Tan ML. 14-deoxy-11,12-didehydroandrographolide exerts its cytotoxic activities by regulating ER-stress related kinases and inducing autophagic-type morphology in T-47D breast carcinoma cells. The 4th International Conference on Natural Products for Health and Beauty, 28th to 30th November 2012, Chiang Mai, Thailand (oral presentation).

32. Koe XF, Lim EL, Seah TC, Amanah A, Adenan MI, Tan ML. The effects of deoxyelephantopin on the CYP3A4 mRNA, protein and enzymatic activities. International Conference and Exhibition of Pharmaceuticals, Nutraceuticals and Cosmeceuticals, 20-21 November 2012, Kuala Lumpur.

33. Abduraman MA, Mohd Nawi MS, Wahab HA, Yusof, R & Tan ML. The development of specific NS2B/NS3 protease inhibition assay using Alphascreen Technology. National Biotechnology Seminar 2013, 6 - 8 Jun 2013, Hotel Equatorial Pulau Pinang.

34. Teah YF, Tan ML. Construction of a stable recombinant Hek293-hERG cell line for use in hERG potassium channel blockade assay. National Biotechnology Seminar 2013, 6- 8 Jun 2013, Hotel Equatorial, Pulau Pinang.

35. Abduraman MA, Mohd Nawi MS, Wahab HA, Yusof R & Tan ML. The development of dengue virus NS2B/NS3 protease inhibition assay for lead compound optimization in drug discovery process, International conference on medical and health sciences, 22-24th May 2013, Renaissance Hotel, Kota Bahru, Kelantan, Malaysia.

36. Teah YF & Tan ML. The establishment of a recombinant HEK293 cell line expressing hERG potassium channels for patch clamp assay. International Conference on medical and health sciences, 22-24th May 2013, Renaissance Hotel, Kota Bahru, Kelantan, Malaysia (Best Poster Presentation).

37.Teah YF, Jamil MF, Adenan MI, Majid, MIA & Tan ML. The effects of mitragynine on hERG current, mRNA and protein expression. Health and Life Sciences Postgraduate Conference, 10-11th June 2014, Renaissance Hotel, Kota Bahru, Kelantan, Malaysia (Best Poster Presentation).

38. Teah YF, Amanah A, Adenan MI, Majid MIA & Tan ML. Development of an in vitro whole-cell patch clamp assay for hERG screening and effects of deoxyelephantopin on hERG inhibition. Pharmacology and Physiology International Scientific Congress 2014, 22-24th August 2014, Putra World Trade Centre, Kuala Lumpur (oral presentation).

39..Tay YL, Wahab, HA. & Tan ML. Development of a recombinant HEK293 cell line expressing hERG1a/1b for hERG1 inhibition screening in whole cell patch clamp assays. Pharmacology & Physiology International Scientific Congress 2014, 22-24 August 2014 2014 Putra World Trade Centre, Kuala Lumpur (oral presentation)

40. Tan HK. & Tan ML. The molecular mechanism of 14-deoxy-11, 12-didehydroandrographolide-induced cell death in T-47D breast carcinoma cells Pharmacology & Physiology International Scientific Congress 2014, 22-24 August 2014 2014 Putra World Trade Centre, Kuala Lumpur (oral presentation)

41.Abduraman MA, Nawi MSM, Hariono M, Wahab HA, Yusof R, Rahman NA & Tan ML. The development of NS2B/NS3 protease inhibition assay using AlphaScreen® Technology and screening of leads for potential anti-dengue cctivity. Pharmacology & Physiology International Scientific Congress 2014, 22-24 August 2014 2014a Putra World Trade Centre, Kuala Lumpur, Malaysia. (poster).

42. Abduraman MA, Nawi MSM, Hariono M, Wahab HA, Yusof R, Rahman NA. & Tan ML. The screening of 6-Thioguanine derivatives activities on dengue virus NS2B/NS3 protease inhibition using AlphaScreen® assa platform. Health and Life Sciences Postgraduate Conference 2014, 10-11 June 2014 2014b Renaissance Hotel, Kota Bharu, Malaysia. (poster).

43. Rusli N, Jamil MF, Adenan MI, Kaur G, Tan ML. The effects of mitragynine on the gene and protein expression of P-gp in Caco-2 cell line. International Anatomical amd Biomedical Scientific Conference (IABS) 18-20^th August 2015. Universiti Putra Malaysia, Selangor, Malaysia (oral presentation)

44. Rusli N, Jamil MF, Adenan MI, Kaur G, Tan ML. The effects of mitragynine on transport activity, mRNA and protein expression of P-glycoprotein in Caco-2 cell line. 2nd AMDI International Biohealth Sciences Conference 2016, Sains@USM, Penang, 27-28 January 2016.(poster).

45. Tay YL, Jamil MF, Adenan MI, Wahab HA & Tan ML. The effects of mitragynine on hERG1a/1b current, mRNA and protein expression. 2nd AMDI International Biohealth Sciences Conference 2016, Sains@USM, Penang, 27-28 January 2016. (oral presentation).

46. Chong, YM & Tan ML. The establishment of a recombinant CHO-K1 cell line expressing human organic anion transporter 1 (hOAT1) for uptake transport assay. 2nd AMDI International Biohealth Sciences Conference 2016, Penang, 27-28 January 2016.(poster).

Publications

1. Tan ML, Tengku Muhammad TS, Samian MR, Najimudin N and Sulaiman SF (2003) Effects of Murdannia japonica (Thunberg) Faden extracts on growth rates of certain cancer cell lines. . 2003; 4, 57-62.

2. Tan ML, Muhammad TS, Najimudin N, Sulaiman SF. Growth arrest and non-apoptotic programmed cell death associated with the up-regulation of c-myc mRNA expression in T-47D breast tumor cells following exposure to Epipremnum pinnatum (L.) Engl. hexane extract. J Ethnopharmacol. 2005 Jan 15;96(3):375-83. PubMed PMID:15619555 (ISI: IF ).

3. Tan ML, Sulaiman SF, Najimuddin N, Samian MR, Muhammad TS. Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line. J Ethnopharmacol. 2005 Jan 4;96(1-2):287-94. PubMed PMID:15588681 (ISI: IF 3.014).

4. Tan ML, Sulaiman SF, Najimudin N, Balakrishnan V, Tengku   Muhammad TS. Cytotoxic and molecular mechanisms of fraction H5 of Epipremnum pinnatum (L.) Engl., a Malaysian Anticancer Herb in Mammary Carcinoma Cells. Annals of Microscopy (S’pore). 2005; 5: 74-84.

5. Tan ML, Kuroyanagi M, Sulaiman SF, Najimudin N, Tengku Muhammad TS. Cytotoxic activities of major diterpenoid constituents of Andrographis paniculata in a panel of human tumor cell lines. Pharmaceutical Biology. 2005; 43(6): 501-508. (ISI: IF 1.046).

6. Tan ML, Sulaiman SF, Tengku Muhammad TS. An Overview of the Cytotoxicity and Molecular Mechanisms of Cell Death of Selected Medicinal Plants with Anticancer Properties, a Malaysian Perspective (Chapter 8) in Recent Progress in Medicinal Plants, Natural Products 2006; Volume 15, Studium Press LLC. USA. (ISBN: 0-9761849-7-4), pp. 149-175.

7. Tan ML, Sulaiman SF, Najimudin N, Tengku Muhammad TS. Anticancer medicinal plant, Epipremnum pinnatum (L.) Engl. chloroform extracts elicited both apoptotic and non-apoptotic cell deaths in T-47D mammary carcinoma cells. KMITL Science and Technology Journal 2007 7(1): 24-43.

8.Moad AI, Lan TM, Kaur G, Hashim H, Mabruk MJ. Immunohistochemical determination of the P15 protein expression in cutaneous squamous cell carcinoma. J Cutan Pathol. 2009 Feb;36(2):183-9. doi: 10.1111/j.1600-0560.2008.00989.x.PubMed PMID: 18564286 (ISI: IF 1.561).

9.Tan ML, Ooi JP, Ismail N, Moad AI, Muhammad TS. Programmed cell death pathways and current antitumor targets. Pharm Res. 2009 Jul;26(7):1547-60. doi:10.1007/s11095-009-9895-1. Review. PubMed PMID: 19407932. (ISI: IF 4.093).

10. Othman EQ, Kaur G, Mutee AF, Muhammad TS, Tan ML. Immunohistochemical expression of MAP1LC3A and MAP1LC3B protein in breast carcinoma tissues. J Clin Lab Anal. 2009;23(4):249-58. doi: 10.1002/jcla.20309. PubMed PMID: 19623642 (ISI: IF 1.384).

11. Mutee AF, Kaur G, Kumar G, Tengku Muhammad TS, Khalid IA, Tan ML. Immunohistochemical evaluation of mTOR and Beclin-1 proteinexpression in human breast cancer and adjacent normal tissues, a study in Malaysian patients. The Open Pathology Journal. 2009; 3: 111-117.

12.Mutee AF, Kaur G, Moad AI, Tan ML, Tengku Muhammad TS. Immunohistochemical expression of mTOR protein in breast carcinoma tissues. The Internet Journal of Laboratory Medicine. 2010; 4 (1).

13. Ooi JP, Kuroyanagi M, Sulaiman SF, Muhammad TS, Tan ML. Andrographolide and14-deoxy-11, 12-didehydroandrographolide inhibit cytochrome P450s in HepG2hepatoma cells. Life Sci. 2011 Feb 28;88(9-10):447-54. doi:

14. Utar Z, Majid MI, Adenan MI, Jamil MF, Lan TM. Mitragynine inhibits the COX-2 mRNA expression and prostaglandin E₂ production induced by lipopolysaccharide in RAW264.7 macrophage cells. J Ethnopharmacol. 2011 Jun 14;136(1):75-82. doi:10.1016/j.jep.2011.04.011. PubMed PMID: 21513785 (ISI : IF 3.014).

15. Ooi KL, Muhammad TS, Tan ML, Sulaiman SF. Cytotoxic, apoptotic andanti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidantisolated from the polyphenolic-rich extract of Elephantopus mollis Kunth. J(ISI : IF 3.014).

16. Tan ML, Tan HK, Oon CE, Kuroyanagi M, Muhammad TS. Identification of genesinvolved in the regulation of 14-deoxy-11,12-didehydroandrographolide-inducedtoxicity in T-47D mammary cells. Food Chem Toxicol. 2012 Feb;50(2):431-44. doi:10.1016/j.fct.2011.11.001. PubMed PMID: 22101062 (ISI: IF 3.010)

17. Moad AI, Tan ML, Kaur G, Mabruk M. Lack of increased P15INK4B proteinexpression in basal cell carcinomas. Asian Pac J Cancer Prev.2012;13(12):6239-44. PubMed PMID: 23464438 (ISI: IF 0.659).

18. Lim EL, Seah TC, Koe XF, Wahab HA, Adenan MI, Jamil MF, Majid MI, Tan ML. In vitro evaluation of cytochrome P450 induction and the inhibition potential ofmitragynine, a stimulant alkaloid. Toxicol In Vitro. 2013 Mar;27(2):812-24. doi: 10.1016/j.tiv.2012.12.014. PubMed PMID: 23274770 (ISI: IF 2.903).

19. Jamil MF, Subki MF, Lan TM, Majid MI, Adenan MI. The effect of mitragynine on cAMP formation and mRNA expression of mu-opioid receptors mediated by chronic morphine treatment in SK-N-SH neuroblastoma cell. J Ethnopharmacol. 2013 Jun 21;148(1):135-43. doi: 10.1016/j.jep.2013.03.078. PubMed PMID: 23608241 (ISI: IF 2.998).

20. Moad AI, Muhammad TS, Oon CE, Tan ML. Rapamycin induces apoptosis whenautophagy is inhibited in T-47D mammary cells and both processes are regulated by(ISI: IF 2.38).

21. Koe XF, Lim EL, Seah TC, Amanah A, Wahab HA, Adenan MI, Sulaiman SF, Tan ML. Evaluation of in vitro cytochrome P450 induction and inhibition activity of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber L. Food Chem Toxicol. 2013 Oct;60:98-108. doi: 10.1016/j.fct.2013.07.030. PubMed PMID:23876819 (ISI IF 3.010).

22. Tan HK, Moad AI, Tan ML. The mTOR signalling pathway in cancer and the potential mTOR inhibitory activities of natural phytochemicals. Asian Pac JCancer Prev. 2014;15(16):6463-75. Review. PubMed PMID: 25169472 (ISI: IF 2.514).

23. Tan ML, Lim LE. The effects of Andrographis paniculata (Burm.f.) Nees extract and diterpenoids on the CYP450 isoforms' activities, a review of possible herb-drug interaction risks. Drug Chem Toxicol. 2015;38(3):241-53. doi: 10.3109/01480545.2014.947504. Review. PubMed PMID: 25156015 (ISI: IF 1.233).

24. Hariono M, Wahab HA, Tan ML, Rosli MM, Razak IA. 9-Benzyl-6-benzyl-sulfanyl-9H-purin-2-amine. Acta Crystallogr Sect E Struct Rep Online. 2014 Feb 12;70(Pt 3):o288. doi: 10.1107/S1600536814001986. eCollection 2014 Mar 1. PubMed PMID: 24764997; PubMed Central PMCID: PMC3998501 (ISI IF 0.35).

25. Tay YL, Wahab HA, Tan ML. In silico interaction of Mitragynine and its analogues with human Ether –a-Go-Go-Related Gene (hERG) channel. Asian Pacific Journal of Tropical Disease 2014; 4 (3) pg 233 (ISI: IF 1.062).

26. Tan ML, Tan HK. Drug-herb interaction studies of herbal drugs and traditional medicine. Asean-NDI Link Newsletter Volume 1, Issue 2, July - September 2014 ISSN 2362-8855.

27. Lu J, Wei H, Wu J, Jamil MF, Tan ML, Adenan MI, Wong P, Shim W. Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes. PLoS One. 2014 Dec 23;9(12):e115648. doi: 10.1371/journal.pone.0115648. eCollection 2014. PubMed PMID: 25535742; PubMed Central PMCID: PMC4275233 (ISI IF 3.234).

28. Koe XF, Tengku Muhammad TS, Chong AS, Wahab HA, Tan ML. Cytochrome P450 induction properties of food and herbal-derived compounds using a novel multiplex RT-qPCR in vitro assay, a drug-food interaction prediction tool. Food Sci Nutr.2014 Sep;2(5):500-20. doi: 10.1002/fsn3.122. Epub 2014 Jun 10. PubMed PMID:25473508; PubMed Central PMCID: PMC4237480.

29. Ooi KL, Loh SI, Tan ML, Muhammad TS, Sulaiman SF. Growth inhibition of human liver carcinoma HepG2 cells and α-glucosidase inhibitory activity of Murdannia bracteata (C.B. Clarke) Kuntze ex J.K. Morton extracts. J Ethnopharmacol. 2015 Mar 13;162:55-60. doi: 10.1016/j.jep.2014.12.030. Epub 2014 Dec 29. PubMed PMID: 25554642 (ISI: IF 2.998).

30. Seah TC, Tay YL, Tan HK, Muhammad TS, Wahab HA, Tan ML. Determination of CYP3A4 Inducing Properties of Compounds Using a Laboratory-Developed Cell-Based Assay. Int J Toxicol. 2015 Sep-Oct;34(5):454-68. doi: 10.1177/ 1091581815599335.Epub 2015 Aug 12. PubMed PMID: 26268769 (ISI: IF1.29).

31. Rusli N, Jamil MF, Adenan MI, Kaur G, Tan ML. The effects of mitragynine on the gene and protein expression of P-gp in Caco-2 cell line.Malaysian Journal of Microscopy 2015; 11(1).

32. Tan ML, Tan HK, Tengku Muhammad TS. (2015) Chapter 13: Apoptosis and Cancer, in Rezaei N (Ed) Cancer Immunology: A Translational Medicine Context, (pp 209-242) Springer-Verlag Berlin Heidelberg.

33. Tan ML, Tan HK, Moad AI, Tengku Muhammad TS. (2015) Chapter 14: Autophagy and Necroptosis in Cancer, in Rezaei N (Ed) Cancer Immunology: A Translational Medicine Context, (pp 243-273) Springer-Verlag Berlin Heidelberg.

34. Klionsky DJ, Tan ML et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3^rd edition). Autophagy. 2016 Jan 2;12(1):1-222. PubMed PMID: 26799652 (ISI: IF 11.753).

35. Tan HK, Muhammad TS, Tan ML. 14-Deoxy-11,12-didehydroandrographolide induces DDIT3-dependent endoplasmic reticulum stress-mediated autophagy in T-47D breast carcinoma cells. Toxicol Appl Pharmacol. 2016 Apr 2;300:55-69. doi:10.1016/j.taap.2016.03.017. [Epub ahead of print] PubMed PMID: 2704911. (ISI: IF 3.71).

36. Tan HK, Tengku Muhammad TS, Tan ML. Cytotoxic data of 14-deoxy-11,12-didehydroandrographolide (14-DDA), double transfection and DDIT3 silencing data in T-47D breast carcinoma cells. Data Brief. 2016 Apr 23;7:1506-10. doi:10.1016/j.dib.2016.04.046. eCollection 2016 Jun. PubMed PMID: 27182548; PubMed Central PMCID: PMC4857397.

37. Tay YL, Teah YF, Chong YM, Jamil MF, Kollert S, Adenan MI, Wahab HA, Döring F, Wischmeyer E, Tan ML. Mitragynine and its potential blocking effects on specific cardiac potassium channels. Toxicol Appl Pharmacol. 2016 Aug 15;305:22-39. doi:10.1016/j.taap.2016.05.022. PubMed PMID: 27260674 (ISI IF 3.71).

38. Tan ML, Lim EL, Koe XF, Tay YL, Teah YF, Rusli N, Jamil MF, Adenan MI, Wahab HA, Wischmeyer E, Wei H. In vitro safety assessments of mitragynine, an euphoric compound isolated from Kratom, a herb-based legal high. Toxicology Letters 2016; 258S, v–vi. (ISI IF 3.262).

Research Grants

Awards

1. Silver and Bronze Medal at the International and Invention Exhibition (ITEX) 2011

2. Silver Medal at the Malaysian Technology Expo (MTE) 2013 - Drug-drug and drug-herb interactions and prediction platform – a safety assessment toolfor standardized herbal preparations and drugs

3. Gold Medal – Bioinnovation Award at the BioMalaysia 2013 - A novel drug-drug and drug-herb interactions prediction platform, a safety assessment tool for drugs and herbal medicine

4. Excellent Staff Award 2014

Appointments

1. Research Fellow of the Malaysian Institute of Pharmaceuticals and Nutraceuticals (IPharm), National Institutes of Biotechnology Malaysia (1 Jan 2012 – current)

2. Committee of the Good Laboratory Practice (MyGLPC, Standards Malaysia, MOSTI) (1 Jan 2010 – 31 December 2014)

3. Expert panel for Dana R,D&C Ministry of Science Technology and Innovation Malaysia grant evaluation (technical and finance)” – October 2011 – current

4. Expert panel for university internal grant evaluation

5.Consultant at Zontron Pharmaceuticals Sdn Bhd, Malaysia

6. Editor for Helliyon Journal, Elsevier

7.Reviewer for ISI journals eg. Food and Chemical Tox, Cellular Physiology and Biochemistry, Toxicology and Pharmacolgy, Drug Metabolism Letters, Oncotarget, etc.

8. Reviewer for National Science Center Poland grant applications

Supervison